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Kinetics
Interaction kinetics
Interaction kinetics describes the interaction between one or more components. After the interaction the components leave each other unchanged as opposed to enzyme kinetics. The interaction kinetics can be subdivided in three distinct phases.
- Association: two or more molecules bind to each other
- Steady state: the amount of molecules that is binding is equal to the amount of bonds that is breaking
- Dissociation: the breaking of the bounds between the molecules
Protein interaction
Each of the three phases contains information over the interaction between the molecules in terms of how fast the association or dissociation is and how strong the overall interaction.
Kinetics
Regeneration
When the dissociation is very slow, it takes a long time before all components are free. Therefore, a solution with for instance low pH or high salt is injected to break the interaction. This fourth phase is referred to as the regeneration.
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